![]() ![]() Fifteen of the examined dogs were on putative, rigorous ML prevention. DNA sequencing of the PCR products confirmed D. immitis specific real-time PCR, the calculated microfilaria concentration ranged from 1 to 44,957 microfilariae/ml and from 7 to 60,526 microfilariae/ml, respectively. Using high resolution melt real-time PCR and D. immitis was detected in the samples from Queensland and New South Wales, Australia. The P-glycoprotein genotype was determined to test whether Australian-sourced heartworm shared the same genetic markers as those suspected of ML-resistance in North America. immitis specific real-time PCR assay, were applied to microfilaria-positive dogs. immitis from Acanthocheilonema reconditum with quantification of microfilariae in canine blood samples, together with D. immitis and real-time PCR for quantification and differentiation between D. Rapid antigen diagnostic tests capable of detection of D. Blood samples from 39 dogs from Queensland and two dogs from New South Wales were investigated for canine filarioids. MethodsĪ hotspot of canine heartworm antigen-positive and microfilaria-positive dogs has been detected recently in Queensland, Australia. The aim of this study was to confirm endemism of the heartworm under current Australian conditions using a cohort of recent microfilaria-positive dogs which were on variable heartworm prevention. ![]() The mainstream prevention of the heartworm is based on macrocyclic lactone (ML) administration. Heartworm ( Dirofilaria immitis) in dogs is considered endemic in Australia, but the clinical heartworm disease caused by the heartworm is rare and prevalence is low. ![]()
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